The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells <i>in vitro</i>

Weiyi Xia; Zheng Ye; Hailing Li; Doulathunnisa Jaffar Ali; Jiahua Ding; Bo Sun; Zhongdang Xiao

doi:doi:10.11648/j.sd.20180601.16

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The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro

Weiyi Xia, Zheng Ye, Hailing Li, Doulathunnisa Jaffar Ali, Jiahua Ding, Bo Sun, Zhongdang Xiao

Published in Science Discovery (Volume 6, Issue 1)

Received: 1 May 2018 Published: 23 May 2018

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Abstract

To investigate the immune-regulatory function of exosomes from human umbilical cord blood plasma on T cells in peripheral blood, differential centrifugation was used to isolate the exosomes from umbilical cord blood plasma (Pexo) firstly. The exosome was characterized by transmission electronic microscope and the western blot of CD63, Lamp2. Exosomes were cultured with peripheral blood lymphocytes which were isolated from the peripheral blood for 72 h, and analyzed for the changes of the Treg subpopulation and memory T cells by flow cytometry, and their effect on lymphocyte proliferation was measured by CFSE assay. The production of IFN-γ, IL-2, TNF-α, TGF-β was detected by qRT-PCR. The results show that Pexo could not activate T cells or proliferate the T cells in vitro, and could not regulate the Treg subpopulation or memory T cells, but the expression of IFN-γ and TNF-α was decreased significantly after the treatment with Pexo within 24h. The exosomes from the umbilical cord blood plasma can decrease the expression of inflammatory factors observably under the condition of keeping the activity and function of the cells. So it has the potential to be used to cure the related immunological diseases due to the inflammatory factors overexpression, especially for the elderly, as their body are usually under the inflammatory state.

Published in	Science Discovery (Volume 6, Issue 1)
DOI	10.11648/j.sd.20180601.16
Page(s)	35-42
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Human Umbilical Cord Blood Plasma, Immune, Exosome, Treg Cells

References

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APA Style

Weiyi Xia, Zheng Ye, Hailing Li, Doulathunnisa Jaffar Ali, Jiahua Ding, et al. (2018). The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro. Science Discovery, 6(1), 35-42. https://doi.org/10.11648/j.sd.20180601.16

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ACS Style

Weiyi Xia; Zheng Ye; Hailing Li; Doulathunnisa Jaffar Ali; Jiahua Ding, et al. The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro. Sci. Discov. 2018, 6(1), 35-42. doi: 10.11648/j.sd.20180601.16

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AMA Style

Weiyi Xia, Zheng Ye, Hailing Li, Doulathunnisa Jaffar Ali, Jiahua Ding, et al. The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro. Sci Discov. 2018;6(1):35-42. doi: 10.11648/j.sd.20180601.16

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@article{10.11648/j.sd.20180601.16,
  author = {Weiyi Xia and Zheng Ye and Hailing Li and Doulathunnisa Jaffar Ali and Jiahua Ding and Bo Sun and Zhongdang Xiao},
  title = {The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro},
  journal = {Science Discovery},
  volume = {6},
  number = {1},
  pages = {35-42},
  doi = {10.11648/j.sd.20180601.16},
  url = {https://doi.org/10.11648/j.sd.20180601.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20180601.16},
  abstract = {To investigate the immune-regulatory function of exosomes from human umbilical cord blood plasma on T cells in peripheral blood, differential centrifugation was used to isolate the exosomes from umbilical cord blood plasma (Pexo) firstly. The exosome was characterized by transmission electronic microscope and the western blot of CD63, Lamp2. Exosomes were cultured with peripheral blood lymphocytes which were isolated from the peripheral blood for 72 h, and analyzed for the changes of the Treg subpopulation and memory T cells by flow cytometry, and their effect on lymphocyte proliferation was measured by CFSE assay. The production of IFN-γ, IL-2, TNF-α, TGF-β was detected by qRT-PCR. The results show that Pexo could not activate T cells or proliferate the T cells in vitro, and could not regulate the Treg subpopulation or memory T cells, but the expression of IFN-γ and TNF-α was decreased significantly after the treatment with Pexo within 24h. The exosomes from the umbilical cord blood plasma can decrease the expression of inflammatory factors observably under the condition of keeping the activity and function of the cells. So it has the potential to be used to cure the related immunological diseases due to the inflammatory factors overexpression, especially for the elderly, as their body are usually under the inflammatory state.},
 year = {2018}
}

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TY  - JOUR
T1  - The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro
AU  - Weiyi Xia
AU  - Zheng Ye
AU  - Hailing Li
AU  - Doulathunnisa Jaffar Ali
AU  - Jiahua Ding
AU  - Bo Sun
AU  - Zhongdang Xiao
Y1  - 2018/05/23
PY  - 2018
N1  - https://doi.org/10.11648/j.sd.20180601.16
DO  - 10.11648/j.sd.20180601.16
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 35
EP  - 42
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20180601.16
AB  - To investigate the immune-regulatory function of exosomes from human umbilical cord blood plasma on T cells in peripheral blood, differential centrifugation was used to isolate the exosomes from umbilical cord blood plasma (Pexo) firstly. The exosome was characterized by transmission electronic microscope and the western blot of CD63, Lamp2. Exosomes were cultured with peripheral blood lymphocytes which were isolated from the peripheral blood for 72 h, and analyzed for the changes of the Treg subpopulation and memory T cells by flow cytometry, and their effect on lymphocyte proliferation was measured by CFSE assay. The production of IFN-γ, IL-2, TNF-α, TGF-β was detected by qRT-PCR. The results show that Pexo could not activate T cells or proliferate the T cells in vitro, and could not regulate the Treg subpopulation or memory T cells, but the expression of IFN-γ and TNF-α was decreased significantly after the treatment with Pexo within 24h. The exosomes from the umbilical cord blood plasma can decrease the expression of inflammatory factors observably under the condition of keeping the activity and function of the cells. So it has the potential to be used to cure the related immunological diseases due to the inflammatory factors overexpression, especially for the elderly, as their body are usually under the inflammatory state.
VL  - 6
IS  - 1
ER  -

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Author Information

Weiyi Xia

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China
Zheng Ye

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China
Hailing Li

Zhongda Hospital, Department of Obstetrics & Gynecology,Southeast University, Nanjing, China
Doulathunnisa Jaffar Ali

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China
Jiahua Ding

Zhongda Hospital, Department of Hematology, Key Deptment Jiangsu Medicine, Southeast University, Nanjing, China
Bo Sun

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China
Zhongdang Xiao

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China

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APA Style

Weiyi Xia, Zheng Ye, Hailing Li, Doulathunnisa Jaffar Ali, Jiahua Ding, et al. (2018). The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro. Science Discovery, 6(1), 35-42. https://doi.org/10.11648/j.sd.20180601.16

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ACS Style

Weiyi Xia; Zheng Ye; Hailing Li; Doulathunnisa Jaffar Ali; Jiahua Ding, et al. The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro. Sci. Discov. 2018, 6(1), 35-42. doi: 10.11648/j.sd.20180601.16

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AMA Style

Weiyi Xia, Zheng Ye, Hailing Li, Doulathunnisa Jaffar Ali, Jiahua Ding, et al. The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro. Sci Discov. 2018;6(1):35-42. doi: 10.11648/j.sd.20180601.16

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@article{10.11648/j.sd.20180601.16,
  author = {Weiyi Xia and Zheng Ye and Hailing Li and Doulathunnisa Jaffar Ali and Jiahua Ding and Bo Sun and Zhongdang Xiao},
  title = {The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro},
  journal = {Science Discovery},
  volume = {6},
  number = {1},
  pages = {35-42},
  doi = {10.11648/j.sd.20180601.16},
  url = {https://doi.org/10.11648/j.sd.20180601.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20180601.16},
  abstract = {To investigate the immune-regulatory function of exosomes from human umbilical cord blood plasma on T cells in peripheral blood, differential centrifugation was used to isolate the exosomes from umbilical cord blood plasma (Pexo) firstly. The exosome was characterized by transmission electronic microscope and the western blot of CD63, Lamp2. Exosomes were cultured with peripheral blood lymphocytes which were isolated from the peripheral blood for 72 h, and analyzed for the changes of the Treg subpopulation and memory T cells by flow cytometry, and their effect on lymphocyte proliferation was measured by CFSE assay. The production of IFN-γ, IL-2, TNF-α, TGF-β was detected by qRT-PCR. The results show that Pexo could not activate T cells or proliferate the T cells in vitro, and could not regulate the Treg subpopulation or memory T cells, but the expression of IFN-γ and TNF-α was decreased significantly after the treatment with Pexo within 24h. The exosomes from the umbilical cord blood plasma can decrease the expression of inflammatory factors observably under the condition of keeping the activity and function of the cells. So it has the potential to be used to cure the related immunological diseases due to the inflammatory factors overexpression, especially for the elderly, as their body are usually under the inflammatory state.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - The Immune-Regulatory Effect of the Exosomes from Human Umbilical Cord Blood Plasma on T Cells in vitro
AU  - Weiyi Xia
AU  - Zheng Ye
AU  - Hailing Li
AU  - Doulathunnisa Jaffar Ali
AU  - Jiahua Ding
AU  - Bo Sun
AU  - Zhongdang Xiao
Y1  - 2018/05/23
PY  - 2018
N1  - https://doi.org/10.11648/j.sd.20180601.16
DO  - 10.11648/j.sd.20180601.16
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 35
EP  - 42
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20180601.16
AB  - To investigate the immune-regulatory function of exosomes from human umbilical cord blood plasma on T cells in peripheral blood, differential centrifugation was used to isolate the exosomes from umbilical cord blood plasma (Pexo) firstly. The exosome was characterized by transmission electronic microscope and the western blot of CD63, Lamp2. Exosomes were cultured with peripheral blood lymphocytes which were isolated from the peripheral blood for 72 h, and analyzed for the changes of the Treg subpopulation and memory T cells by flow cytometry, and their effect on lymphocyte proliferation was measured by CFSE assay. The production of IFN-γ, IL-2, TNF-α, TGF-β was detected by qRT-PCR. The results show that Pexo could not activate T cells or proliferate the T cells in vitro, and could not regulate the Treg subpopulation or memory T cells, but the expression of IFN-γ and TNF-α was decreased significantly after the treatment with Pexo within 24h. The exosomes from the umbilical cord blood plasma can decrease the expression of inflammatory factors observably under the condition of keeping the activity and function of the cells. So it has the potential to be used to cure the related immunological diseases due to the inflammatory factors overexpression, especially for the elderly, as their body are usually under the inflammatory state.
VL  - 6
IS  - 1
ER  -

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