Rolling circle amplification (RCA) of genomic or plasmid DNA has found wide used in technique academic and biotechnology reseach. In this study, we have successfully found what caused the nonspecific product in the reaction, thus designing an improved RCA which overcomes false priming at sites where primer dimers are formed. The results demonstrated that PEG or PEI could interact with the components of the improved RCA system, and help increase the reaction rate and yield of the improved RCA system, and dramatically reduces the nonspecific RCA products. This low-cost and efficient method dramatically overcomes nonspecific product amplification phenomenon in the RCA reaction which is problematic and has existed for a long time.
| Published in | Science Discovery (Volume 5, Issue 6) |
| DOI | 10.11648/j.sd.20170506.28 |
| Page(s) | 496-501 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
Rolling Circle Amplification, Recombinant Plasmid, Polyetherimide, Polyethylene Glycol
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APA Style
Yun Wei Zhang, Dan Hou, Wei Dong Xiao, Jenni Firrman, Lin Shu Liu, et al. (2017). Method for Improving Rolling Circle Amplification Sensitivity and Specificity. Science Discovery, 5(6), 496-501. https://doi.org/10.11648/j.sd.20170506.28
ACS Style
Yun Wei Zhang; Dan Hou; Wei Dong Xiao; Jenni Firrman; Lin Shu Liu, et al. Method for Improving Rolling Circle Amplification Sensitivity and Specificity. Sci. Discov. 2017, 5(6), 496-501. doi: 10.11648/j.sd.20170506.28
AMA Style
Yun Wei Zhang, Dan Hou, Wei Dong Xiao, Jenni Firrman, Lin Shu Liu, et al. Method for Improving Rolling Circle Amplification Sensitivity and Specificity. Sci Discov. 2017;5(6):496-501. doi: 10.11648/j.sd.20170506.28
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Download@article{10.11648/j.sd.20170506.28,
author = {Yun Wei Zhang and Dan Hou and Wei Dong Xiao and Jenni Firrman and Lin Shu Liu and Yong Diao and Zhi Qing Qi},
title = {Method for Improving Rolling Circle Amplification Sensitivity and Specificity},
journal = {Science Discovery},
volume = {5},
number = {6},
pages = {496-501},
doi = {10.11648/j.sd.20170506.28},
url = {https://doi.org/10.11648/j.sd.20170506.28},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20170506.28},
abstract = {Rolling circle amplification (RCA) of genomic or plasmid DNA has found wide used in technique academic and biotechnology reseach. In this study, we have successfully found what caused the nonspecific product in the reaction, thus designing an improved RCA which overcomes false priming at sites where primer dimers are formed. The results demonstrated that PEG or PEI could interact with the components of the improved RCA system, and help increase the reaction rate and yield of the improved RCA system, and dramatically reduces the nonspecific RCA products. This low-cost and efficient method dramatically overcomes nonspecific product amplification phenomenon in the RCA reaction which is problematic and has existed for a long time.},
year = {2017}
}
TY - JOUR T1 - Method for Improving Rolling Circle Amplification Sensitivity and Specificity AU - Yun Wei Zhang AU - Dan Hou AU - Wei Dong Xiao AU - Jenni Firrman AU - Lin Shu Liu AU - Yong Diao AU - Zhi Qing Qi Y1 - 2017/12/28 PY - 2017 N1 - https://doi.org/10.11648/j.sd.20170506.28 DO - 10.11648/j.sd.20170506.28 T2 - Science Discovery JF - Science Discovery JO - Science Discovery SP - 496 EP - 501 PB - Science Publishing Group SN - 2331-0650 UR - https://doi.org/10.11648/j.sd.20170506.28 AB - Rolling circle amplification (RCA) of genomic or plasmid DNA has found wide used in technique academic and biotechnology reseach. In this study, we have successfully found what caused the nonspecific product in the reaction, thus designing an improved RCA which overcomes false priming at sites where primer dimers are formed. The results demonstrated that PEG or PEI could interact with the components of the improved RCA system, and help increase the reaction rate and yield of the improved RCA system, and dramatically reduces the nonspecific RCA products. This low-cost and efficient method dramatically overcomes nonspecific product amplification phenomenon in the RCA reaction which is problematic and has existed for a long time. VL - 5 IS - 6 ER -
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